簡介:艾睿生物提供15P-1細(xì)胞(1411D)實時價格和產(chǎn)品詳細(xì)說明,敬請來電咨詢!詳細(xì)介紹15P-1細(xì)胞(1411D)。
詳細(xì)說明:
15P-1細(xì)胞(1411D)——含 量:99 產(chǎn)品規(guī)格:100000-1000000cells/ml 產(chǎn)品包裝:液體瓶裝 品 牌:ATCC細(xì)胞庫、ScienCell實驗室等細(xì)胞提供單位 產(chǎn) 地:美國、中國
細(xì)胞規(guī)格:100000-1000000cells/ml
儲存:液氮(凍存)或復(fù)蘇培養(yǎng)
運輸:干冰 用途:細(xì)胞領(lǐng)域科學(xué)研究
15P-1細(xì)胞(1411D)于原代凍存或復(fù)蘇培養(yǎng)(這個細(xì)胞提供的周期會長點,少則半個月,多則一兩個月)。每管含有細(xì)胞數(shù)>5×100000cells/ml,此細(xì)胞通過**熒光染色驗證,經(jīng)測試不含有HIV-1、HBV、HCV、支原體、**、酵母和**。細(xì)胞可以達(dá)到10倍增。 公司長期提供細(xì)胞產(chǎn)品,很多細(xì)胞科研單位都特意訂購我公司X細(xì)胞,經(jīng)常購買我們公司細(xì)胞產(chǎn)品的單位有:天津醫(yī)科大學(xué)、寧夏醫(yī)科大學(xué)、**軍醫(yī)大學(xué)、徐州醫(yī)學(xué)院、哈爾濱醫(yī)科大學(xué)、廣州中醫(yī)藥大學(xué)、第三軍醫(yī)大學(xué)、上海交大醫(yī)學(xué)院、蘭州大學(xué)、貴州省遵義市遵義醫(yī)學(xué)院、湖北武漢同濟(jì)醫(yī)院神經(jīng)外科、江蘇省人民醫(yī)院、北京大學(xué)人民醫(yī)院等單位的重點實驗室。
細(xì)胞凍存和復(fù)蘇實驗原理:細(xì)胞凍存及復(fù)蘇的基本原則是慢凍快融,實驗證明這樣可以*大限度的保存細(xì)胞活力。目前細(xì)胞凍存多采用甘油或二甲基亞砜作保護(hù)劑,這兩種物質(zhì)能提高細(xì)胞膜對水的通透性,加上緩慢冷凍可使細(xì)胞內(nèi)的水分滲出細(xì)胞外,減少細(xì)胞內(nèi)冰晶的形成,從而減少由于冰晶形成造成的細(xì)胞損傷。復(fù)蘇細(xì)胞應(yīng)采用快速融化的方法,這樣可以保證細(xì)胞外結(jié)晶在很短的時間內(nèi)即融化,避免由于緩慢融化使水分滲入細(xì)胞內(nèi)形成胞內(nèi)再結(jié)晶對細(xì)胞造成損傷。
細(xì)胞凍存和復(fù)蘇實驗注意事項:
1. 從增殖期到形成致密的單層細(xì)胞以前的培養(yǎng)細(xì)胞都可以用于凍存,但*好為對數(shù)生長期細(xì)胞。在凍存前***好換一次培養(yǎng)液。
2. 將凍存管放入液氮容器或從中取出時,要做好防護(hù)工作,以免**。
3. 凍存和復(fù)蘇*好用新配制的培養(yǎng)液。
人肺動脈平滑肌細(xì)胞凍存和復(fù)蘇的原則:慢凍快融
當(dāng)細(xì)胞冷到零度以下,可以產(chǎn)生以下變化:細(xì)胞器脫水,細(xì)胞中可溶性物質(zhì)濃度升高,并在細(xì)胞內(nèi)形成冰晶。
如果緩慢冷凍,可使細(xì)胞逐步脫水,細(xì)胞內(nèi)不致產(chǎn)生大的冰晶;相反,結(jié)晶就大,大結(jié)晶會造成細(xì)胞膜、綱胞器的損傷和破裂。復(fù)蘇過程應(yīng)快融,目的是防止小冰晶形成大冰晶,即冰晶的重結(jié)晶。
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